Journal: bioRxiv

Article Title: Keratin 5 marks cancer-propagating cells sustained by an osteopontin-producing niche in high-grade serous ovarian carcinoma

doi: 10.64898/2026.01.28.702332

Figure Lengend Snippet: A and B , Organoids derived from SKOV3 cells expressing both lentiviruses (A, GFP and mCherry, orange) or GFP alone (B, green). Scale bar, 100 µm. C , Quantification of KRT5+ (blue symbols, pink bars) and KRT5- (pink symbols, yellow bars) cancer organoids in 6 consecutive passages. All error bars denote s.d. D, Volume of tumors formed by serially diluted (1 x 10 5 , 1 x 10 4 , 1 x 10 3 ) of KRT5+ and KRT5- cells after their s.c. transplantation into different flanks of NSG mice. KRT5-group did not form tumors. E and F , mCherry (E) and KRT5 (F) expression in KRT5+ cell derived xenografts. Elite ABC method. Hematoxylin counterstaining. Scale bar, 60 µm. All error bars denote s.d. G. Live microscopy of cells were isolated by FACS based on their expression of GFP (green) and mCherry (magenta) after coinfection with Lenti-UbC-GFP and Lenti-KRT5mCherry. Orange, Overlay. Individual frames of live microscopy. Scale bar, 60 µm.

Article Snippet: Human ovarian cancer cell lines SKOV3 (ATCC, HTB-77), CAOV3 (ATCC, HTB-75), and CAOV4 (ATCC, HTB-76) were obtained from the American Type Culture Collection.

Techniques: Derivative Assay, Expressing, Transplantation Assay, Microscopy, Isolation

Journal: bioRxiv

Article Title: Keratin 5 marks cancer-propagating cells sustained by an osteopontin-producing niche in high-grade serous ovarian carcinoma

doi: 10.64898/2026.01.28.702332

Figure Lengend Snippet: A and B. Quantification of cells expressing KRT5 and/or SPP1 cells within human HGSC cases from single-cell RNA sequencing. Significance by Mann-Whitney U test. C. RT-PCR analysis of SPP1 expression in KRT5+ and KRT5- subpopulations of SKOV3 cells. D and E, KRT5 (green) and OPN (red) expression in HGSC (D) and primary HGSC organoid (E). Double immunofluorescence, counterstaining with DAPI (blue). Scale bar, (D) 60 µm, E (40 µm). F and G , OPN treatment increases frequency (F) and size (G) of HGSC organoids (n=3). H - K . Effect of cisplatin on frequency (H and J) and size (I and K) of organoids either transduced with SPP1 shRNA (H and I) or treated with OPN (I and K). All organoids were measured 72 hours after treating with cisplatin at different concentrations. All error bars denote s.d.

Article Snippet: Human ovarian cancer cell lines SKOV3 (ATCC, HTB-77), CAOV3 (ATCC, HTB-75), and CAOV4 (ATCC, HTB-76) were obtained from the American Type Culture Collection.

Techniques: Expressing, RNA Sequencing, MANN-WHITNEY, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Transduction, shRNA

Journal: Journal of Translational Medicine

Article Title: Preclinical synergistic effects when combining a radiolabelled HER2-targeting single domain antibody with PARP inhibitor Olaparib

doi: 10.1186/s12967-025-07572-2

Figure Lengend Snippet: Flow cytometry evaluation of HER2 expression in DLD1, DLD1 BRCA2 −/− , and SKOV3 cells. Histogram plots showing HER2 expression (purple) in the cells. The control peak was obtained using an isotype control antibody (gray). (Error bars are depicted as SD)

Article Snippet: Human SKOV3 ovarian cancer cells (RRID: CVCL_0532, ATCC), human colorectal DLD-1 cancer cells (RRID: CVCL_0248, Horizon Discovery) and human colorectal DLD-1 BRCA2 −/− cancer cells (RRID: CVCL_HD57, Horizon Discovery) were cultured in RPMI1640 medium (Gibco).

Techniques: Flow Cytometry, Expressing, Control